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3 edition of The physical mapping of bacteriophage T5 transfer ribonucleic acids found in the catalog.

The physical mapping of bacteriophage T5 transfer ribonucleic acids

Mann-jy Chen

The physical mapping of bacteriophage T5 transfer ribonucleic acids

by Mann-jy Chen

  • 390 Want to read
  • 15 Currently reading

Published .
Written in English


Classifications
LC ClassificationsMicrofilm 45834
The Physical Object
FormatMicroform
Paginationvi, 106 l.
Number of Pages106
ID Numbers
Open LibraryOL1367830M
LC Control Number92895126

Introduction. Detailed restriction maps of a number of viral genomes (Roberts ) have played an important role in the recent analyses of these r, a detailed physical map has not yet been determined for one of the most intensively investigated bacterial viruses, bacteriophage by: The first viruses identified to encode their own set of tRNAs have been the bacteriophage T4 which possesses eight tRNA genes and the bacteriophage T5 carrying at least 14 different tRNAs [24,26,27,28,29,30]. The occurrence of tRNA genes is not restricted to bacteriophages; tRNAs are widespread in different families of by: 8.

Attempts to determine transcription errors during the formation of fluorouracil-containing messenger ribonucleic acid. Biochemistry 5, () Location of single-strand interruptions in the DNA of bacteriophage T5. Proc. Natl. Acad. G.S. and Bujard, H. () Physical mapping of the HindIII, EcoRI, Sal I and SmaI restriction. Berg D, Chamberlin M. Physical studies on ribonucleic acid polymerase from Escherichia coli B. Biochemistry. Dec 22; 9 (26)– [Google Scholar] Brunel F, Davison J. Restriction insensitivity in bacteriophage T5. III. Characterization of EcoRI-sensitive mutants by restriction analysis. J Mol Biol. Mar 15; (4)–

Start studying Chapter 7: Genetic Transfer and Mapping in Bacteria & Bacteriophages. Learn vocabulary, terms, and more with flashcards, games, and other study tools. The sequence of the leuX-coded tRNA is compared with the sequences of the four remaining tRNALeu isoacceptors of E. coli and with two tRNALeu species from bacteriophage T4 and T5.


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The physical mapping of bacteriophage T5 transfer ribonucleic acids by Mann-jy Chen Download PDF EPUB FB2

The locations of cleavage sites, produced by 13 restriction endonucleases, were mapped on the DNA of bacteriophage T5.

Single- and double-digest fragment sizes were determined by agarose gel electrophoresis, using restriction fragments of phi X DNA Cited by: The Physical Mapping of Bacteriophage T5 Transfer tRNAs* (Received for publication, J ) MANN-JY CHEN, JOSEPH LOCKER, AND SAMUEL B.

WEISS From The Franklin McLean Memorial Research Institute, the Departments of Biochemistry. The physical mapping of bacteriophage T5 transfer tRNAs.

Chen MJ, Locker J, Weiss SB. Transfer RNAs, isolated from Escherichia coli F cells infected with T5 bacteriophage, were charged with radioactive amino acids and used in RNA-DNA hybridization studies to detect and locate T5 tRNA cistrons in the T5 DNA by:   Sequence analysis of two DNA fragments generated from bacteriophage T5 DNA by restriction with Hpa I and Hae III has resulted in the detection and localization of nine tRNA genes (His, two Ser genes, Leu, Val, Lys, fMet, Pro, and Ile).

The genes which code for tRNAs His and Leu are partials, whereas the remaining genes are by: Bacteriophages T5 and BF23 have been the two most extensively investigated of the T5 group, which also includes bacteriophages PB, BG3, and 29 alpha. Unusual features of T5 Cited by: Identification of multiple molecular species of bacteriophage T7 lysozyme messenger ribonucleic acid.

Biochemistry13 (16), DOI: /bia Gordon L. Koch. Tyrosyl transfer ribonucleic acid synthetase from Bacillus stearothermophilus. Preparation and properties of the crystallizable enzyme. Introduction. The physical mapping of bacteriophage T5 transfer ribonucleic acids book T5 is a lytic phage that infects enterobacteria Escherichia coli.

It is one of the best-characterized phage in the T5-like viruses genus of the Siphoviridae Family. Not much progress has been reported since McCorquodale and Warner () reviewed the work on bacteriophage T5 and related phages.

The linear and double-stranded DNA genome is nicked at specific Cited by: A general method for the separation of isoaccepting transfer ribonucleic acids: Purification of five leucine transfer ribonucleic acids from Escherichia coli.

Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis(4), DOI: /(71)Cited by: This physical map was aligned with the genetic map. The T4 early regions were further identified by hybridization of RNA synthesized in vitro to the restriction fragments and two promoter regions were localized by filter binding tests and R-loop by: 6.

A detailed molecular dissection of the yeast mitochondrial genome can be made with restriction endonucleases that generate site-specific cuts in DNA. The ordering of restriction fragments provides the basis of the physical mapping of mitochondrial transcripts and antibiotic resistance (ant®) loci, and is a means of analyzing the molecular organization of mtDNA of petite and mit- deletion Cited by: 8.

A transcriptional map of bacteriophage T5 was constructed by measuring the hybridization of radioactive T5 RNA synthesized in vivo with specific DNA fragments, obtained by electrophoretic fractionation of denatured T5 DNA.

For various segments of the T5 DNA molecule, the length of the functional DNA corresponding to each of the three classes of T5 RNA, “pre-early,” “early,” and “late Cited by: 2. Physical map of the dispensable region of the chromosome of BF23 On the basis of the experimental results described in the preceding sections the physical structure of the dispensable region of BF23 DNA was determined and the results are summarized in Fig.

by: 5. Mol. Biol. ()Restriction Insensitivity in Bacteriophage T5 lilt. Characterization of oRI-sensitive Mutants by Restriction Analysis FRANISE BBUNEL AND JOHN DAVISON Unit of Molecular Biology Institute of Cellular Pathology Avenue Hippocrate, 75, B Brussels Belgium (Received 22 Mayand in revised form S November } Despite the fact that its DNA carries Cited by: Optically detected magnetic resonance of Escherichia coli glutamic acid specific transfer ribonucleic acid and its anticodon-anticodon complex with yeast phenylalanine-specific transfer ribonucleic acid.

Biochemistry23 (26), DOI: /biaCited by: Bacteriophage (phage) are obligate intracellular parasites that multiply inside bacteria by making use of some or all of the host biosynthetic machinery (i.e., viruses that infect bacteria.).

There are many similarities between bacteriophages and animal cell viruses. In this article we confirm that pb5 is encoded by the T5 oad gene and describe the isolation, expression, and sequencing of this gene. In order to locate oad precisely, we analyzed recombinants between BF23, a T5-related phage with a different host range, and plasmid clones containing segments of the T5 by:   (1) We have derived a fine-structure map of the 70 kb mitochondrial genome of the yeast S.

cerevisiae, strain DB, and compared it with our previous maps for strain MHB. Restriction fragment maps for 56 enzyme recognition sites for 13 endonucleases, Eco RI, Hpa I, Bam HI, Hha I, Hinc II, Xba I, Hind III, Bgl II, Pvu II, Sal I, Pst I, Sst I, and Xho I, have been derived.

We have used Cited by: Characterization of the rapidly reassociating deoxyribonucleic acid of Cucurbita pepo and the sequences complementary to ribosomal and transfer ribonucleic acids. Biochemistry12 (14), DOI: /bia Richard E. Wettenhall, Ira G. Wool, and Corinne C. by: DNA Replication of Bacteriophage T5.

Fractionation of Intracellular T5 DNA by Agarose Gel Electrophoresis. all of the ends of the mature DNA fall within 20% of each other on the physical. Bacteriophage T5 Transfer RNA ISOLATION AND CHARACTERIZATION OF tRNA SPECIES AND REFINEMENT OF THE tRNA GENE MAP** (Received for publication, Octo ) Clayton Hunt,g Suresh M.

Desai, Judith Vaughan, and Samuel B. Weiss From IThe Franklin McLean Memorial Research Institute, The Departments of Biochemistry and Microbiology, and The.

Vibrio eltor phage e 4 codes for five different tRNA species (S. Chattopadhyay and R. K. Ghosh,Virology,–).The tRNA genes contained in a kb Kpnl fragment (S. Chattopadhyay and R.

K. Ghosh,Virology,–) have been cloned in pUC 19 at the Kpnl site. Two recombinant plasmids, pSR and pSR, produced four of the five tRNA species (arginine, isoleucine Author: Samit Chattopadhyay, Ranajit Kumar Ghosh. We present a linearized physical map of the genome of bacteriophage T4.

This map contains the cleavage sites for restriction enzymes SmaI, KpnI, SalI, BglII, XhoI, XbaI, ClaI, HaeII, EcoRI, and EcoRV.

It also contains about TaqI sites. The promoter sites recognized in vitro and a number of rho independent terminators have also been by: Enterobacteria phage λ (lambda phage, coliphage λ, officially Escherichia virus Lambda) is a bacterial virus, or bacteriophage, that infects the bacterial species Escherichia coli (E.

coli).It was discovered by Esther Lederberg in The wild type of this virus has a temperate life cycle that allows it to either reside within the genome of its host through lysogeny or enter into a lytic Family: Siphoviridae.